In vitro, myelin basic protein (MBP) is a substrate for phosphorylation by several different protein kinases including MAPK, PKA, calmodulin-dependent protein kinase, PKC and phosphorylase kinase. Even highly specific protein kinases such as Raf1, MEK and MEKK can utilize MBP as an alternative substrate. Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis. Applications: Suitable for use in Protein Kinase Assay and radioactive kinase assays. Other applications have not been tested. Protein Kinase Assay: Phosphorylated in a radioactive kinase assay using MAP Kinase 2, Recombinant, Mouse (M2362-06). 32P-phosphorylated, biotinylated MBP was captured using Streptavidin (Agarose) (S7973-98). Storage and Stability: Stable for 6 months after receipt at 4C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Purified using FPLC liquid chromatography and dephosphorylated using Lambda protein phosphatase. Lambda protein phosphatase was inactivated, in the presence of 10mM sodium EDTA, at 65C for one hour. Purity was assessed at 95% using SDS-PAGE and Coomassie blue staining of 1ug of product.
Formulierung:
Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
