| In normal tissues and in a variety of myeloproliferative disorders, myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. It is readily detected in myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythro-leukemia, myeloblastomas, and other hematopoietic disorders. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. Cells of monocytic derivation reveal variable reactivity and are typically weakly positive or nonreactive. Cells comprising the infiltrate of a spectrum of lymphoid malignancies, e.q., lymphoblastic lymphoma or leukemia, chronic lymphocytic leukemia, hairy cell leukemia, non-Hodgkins lymphomas of T-or B-cell type, and Hodgkins disease, are nonreactive, as are the non-neoplastic tissues present in these specimens, except for occasional cells of myeloid derivation. MPO is not observed in the neoplastic cells of a wide variety of epithelial tumors and sarcomas, or in the contiguous non-neoplastic tissues. MPO is useful in differentiating between myeloid and lymphoid leukemias. E.C.: 1.11.1.7 Source: Human neutrophil Purity: 96% (SDS-PAGE), Purified by column chromatography. Appearance: Lyophilized powder from sodium acetate, pH 6.0. Reconstitute with 100ul or 1ml sterile PBS. Concentration: ~1mg/ml (after reconstitution) Specific Activity: As Reported. Definition: One unit of Myeloperoxidase will catalyze the consumption of one micromole of hydrogen peroxide and the production of micromole of tetraguaiacol per minute at pH 7.0 and 25C. Rz Value: 0.8 (A430/A280) Solubility: Green, clear solution (1mg/ml 0.05M phosphate, pH 7.0.) Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
