Nematode Growth Medium (NGM) (Powder)

Produktübersicht

• Artikelname: Nematode Growth Medium (NGM) (Powder)
• Artikelnummer: USB-N1000
• Hersteller Artikelnummer: N1000
• Alternativnummer: USB-N1000-500,USB-N1000-1
• Hersteller: US Biological
• Kategorie: Biochemikalien

Produktbeschreibung

A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58C water bath. N1000 solution needs to completely cool to the desired temperature (58C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58C, place flask(s) onto stir plate(s). Maintain temperature at 58C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution Allow the solution to mix for five minutes before starting to pour plates to ensure that a homogeneous mixture has been achieved. *1M Phosphate buffer is prepared by dissolving 108.3g KH2PO4 and 35.6g K2HPO4 in ddH2O. QS to 1L).