Recombinant protein corresponding to Phe22-Lys644 from human Neuropilin 1, expressed in NS0 cells.
Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibody may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
