Neomycin Phosphotransferase II (NPT II) (APC), Rabbit

Produktübersicht

• Artikelname: Neomycin Phosphotransferase II (NPT II) (APC), Rabbit
• Artikelnummer: USB-N2008-05-APC
• Hersteller Artikelnummer: N2008-05-APC
• Alternativnummer: USB-N2008-05-APC-100
• Hersteller: US Biological
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FLISA, WB
• Immunogen: Purified Neomycin phosphotransferase II.

Produktbeschreibung

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Western Blot analysis of transfected cell lysates with anti-NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids. Applications: Suitable for use in FLISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Produkteigenschaften

• Reinheit: Purified
• Formulierung: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).