Neuraminidase, Clostridium perfringens (Sialidase, nanH, NA)

Artikelnummer: USB-N2100
Artikelname: Neuraminidase, Clostridium perfringens (Sialidase, nanH, NA)
Artikelnummer: USB-N2100
Hersteller Artikelnummer: N2100
Alternativnummer: USB-N2100-5,USB-N2100-10,USB-N2100-25
Hersteller: US Biological
Kategorie: Molekularbiologie
Neuraminidase (sialidase) splits off N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins and are important tools for the study of glycoproteins in protein chemistry and cell biology (Hatton et al. 1973). Chromatographic purification has been developed in this laboratory based on work of Cassidy et al. (1965). Wooley and Gommi (1966) describe the use of this neuraminidase in a method for measuring serotonin receptors. It is also employed to determine bound N-acetylneuraminic acid in tissues. Considerable interest has been shown in using neuraminidase inhibitors as possible anti-viral and anti-bacterial agents. Form: Supplied as a lyophilized powder, 50% sucrose Concentration: ~20u/mg Protein Content (%): 30% Units/Protein (u/mg): 5u/mg Protein (dry weight) A280 mg/ml: ~0.4 Assay Method: The assay is based on the measurement of sialic acid (NANA) released from bovine submaxillary mucin. One unit causes the release of one micromole of sialic acid per minute at 37C and pH 5.0, from bovine submaxillary mucin under the specified conditions. Storage and Stability: Product is stable for 6 months after receipt at 4C.
Reinheit: Chromatographically purified.
Formulierung: Supplied as a lyophilized powder in 50% sucrose