MaxLight(TM) 490 is a new Blue-Green photostable dye conjugate comparable to DyLight(TM)488, Alexa Fluor(TM)488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm), Emission (515nm), Extinction Coefficient 73,000. Nectin-1, also called PVRL1, PRR1, Vec C, CD111, HveC and HigR, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic cells, neuronal cells, endothelial and epithelial cells. Nectin-1 belongs to a new family of immunoglobulin-like molecules that includes four members (Nectin-1, -2, -3 and CD155). Nectin-1 gene is localized to human chromosome 11q23-q24, and encodes at least two different transmembrane isoforms. Nectin-1 is a pan alpha herpes virus entry receptor that mediates entry of many HSV1 and HSV-2 strains, bovine Herpes virus1 (BHV-1) and porcine pseudorabies virus (PRV). Applications: Suitable for use in Immunoprecipitation, Western Blot, Flow Cytometry and FLISA. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation (Native): 5-10ug/reaction Western Blot: 0.1-1ug/ml Flow Cytometry: 5-10ug/ml FLISA: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[6A139]
Reinheit:
Purified by Protein A affinity chromatography
Formulierung:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight(TM)490.
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