ImmunoTek(TM) p-Nitrophenyl Phosphate (pNPP)

Artikelnummer: USB-N2600
Artikelname: ImmunoTek(TM) p-Nitrophenyl Phosphate (pNPP)
Artikelnummer: USB-N2600
Hersteller Artikelnummer: N2600
Alternativnummer: USB-N2600-250,USB-N2600-500
Hersteller: US Biological
Kategorie: Molekularbiologie
Applikation: ELISA
ImmunoTek(TM) p-Nitrophenyl Phosphate (pNPP) p-NPP is a single component reagent system for microwell assay of Calf Intestine Alkaline Phosphatase (CIAP or CAP) labeled probes and is an excellent substrate for phosphatase based ELISA assays. p-NPP produces a soluble end product which is yellow in color and read at 405-410 nm. Both kinetic and end point determinations can be performed. p-NPP is the customary phosphate ester used as a substrate for measuring AP activity due to its high solubility in alkaline buffers which affords zero order kinetics and significant molar extinction coefficient of 18600 mol-1cm-1. Storage and Stability: Stable for 12 months after receipt at 4C. Also Available: N2600: p-Nitrophenyl Phosphate (pNPP) N2600-10: p-Nitrophenyl Phosphate (pNPP), 50X Concentrate, 0.19M/Liter
Formulierung: Supplied as a liquid in 0.95M DEA, pH 9.0