22aa peptide sequence mapping to the 1st extracellular domain of rat NET. The peptide shows no significant homology with other known proteins. Species Sequence Homology: Mouse - 100%, Human, bovine, monkey - 90%, Chicken - 85%.
Noradrenaline transporter or epinephrine transporter (NET or NTT1 or NAT1 or SLC6A2) gene (chromosome 16) encodes a protein of 617aa (mouse 617aa, ~94% identity), with 12-13 transmembrane domains, and cytoplasmic N and C-termini. Expression of the cDNA clone in transfected HeLa cells indicated that noradrenaline transport activity is sodium-dependent and sensitive to selective noradrenaline transport inhibitors. NET mRNA has been localized to the brain stem and adrenal. The predicted protein sequence demonstrated significant amino acid identity with the Na(+)/gamma-aminobutyric acid transporter, thus identifying a new gene family for neurotransmitter transport proteins. Alternative splicing of NET mRNA produces two transporter isoforms, rNETa and rNETb, which differ at their COOH termini. The rNETa isoform reveals a COOH terminus homologous to human NET and transports norepinephrine. In contrast, rNETb revealed no detectable transport function, but reduced functional expression of rNETa when both isoforms were expressed in the same cell. Defects in NET are a cause of orthostatic intolerance (OI), a syndrome characterized by lightheadedness, fatigue, altered mentation and syncore. It is associated with postural tachycardia. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000, using 50-100ng immunogen peptide/well. Western Blot: 1-10ug/ml (ECL) Optimal dilutions to be determined by the researcher. Control Peptide: N3950-06 Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified by affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide.
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