NOS I, Human, Control Peptide (Nitric Oxide Synthase I, bNOS, nNOS, NOS-I)

Produktübersicht

• Artikelname: NOS I, Human, Control Peptide (Nitric Oxide Synthase I, bNOS, nNOS, NOS-I)
• Artikelnummer: USB-N5350-05D
• Hersteller Artikelnummer: N5350-05D
• Alternativnummer: USB-N5350-05D-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: ELISA

Produktbeschreibung

Control peptide for N5350-05D and N5350-05M. N5350-05D is a synthetic peptide corresponding to amino acid sequence 724-739 of human brain NOS. Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues. NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS , I-III, ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~50%. Species Crossreactivity: Human (1433aa), rat (1429aa), mouse (1429aa) and rabbit (1). No significant sequence homology is seen with NOS-2/NOS-3 or other proteins. Applications: Suitable for use in ELISA and Antibody Competition Studies.. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilution: ELISA: Coated at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Highly purified
• Formulierung: Supplied as a liquid in PBS, pH 7.5.