| Control Peptide for O3003-25 (antiserum) and O3003-29 (affinity purified) A 15aa Peptide internal sequence (middle regions) of human OATP Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called renal secretion. Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides, oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Rat Oatp, now called oatp1, encodes a protein of 670 aa. Oatp1 was localized at the apical portions of the S3 segment of the proximal tubule, and at the apical surface of the choroid plexus of rat brain. It is also found in liver, lung, skeletal muscle, and colon. It mediates Na+-independent uptake of wide range of amphipathic substrates including sulfobromopthalein (BSP), bile acids, estrogen conjugates, and neutral steroids, etc. Human liver OATP (670 aa) is only 67% homologous with rat oatp1. It differs in functional properties from rat oatp1 and highly expressed in brain. |
