Control Peptide for O7501 (antiserum) and O7501-05 (affinity purified) Synthetic peptide, 13aa, amino terminus of murine Orexin B. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (1-2). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Preproorexin is 130 amino acid long peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (1-2). These neuropeptides bind and activate two closely related Orexin receptors--G-protein coupled receptors (GPCRs) OX1R and OX2R. Mouse control peptide sequence is 100% homologous in mouse, pig, bovine, dog, rat and 92 % human Orexin-B. Applications: Suitable for use as a control in ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified.
Formulierung:
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
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