Synthetic peptide corresponding to a sequence at the C-terminus of human P2RX3 (385-397aa KQSTDSGAFSIGH), different from the related mouse and rat sequences by one amino acid.
Purinergic receptor P2X, ligand-gated ion channel, 3, also known as P2X3, is a human gene. Its gene is mapped to 11q12. P2X purinoceptors are 397 to 492aa long and have a predicted structure of 2 short intracellular domains, 2 transmembrane-spanning regions, and a large extracellular domain.The P2X3 subunit has 43% and 47% identity with P2X1 and P2X2, respectively, 10 cysteine residues are conserved in all 3 subtypes, so that tertiary structures may also be conserved.1 P2X3 was the only ligand-gated channel known to be expressed exclusively by a subset of sensory neurons. The remarkable selectivity of expression of the channel, coupled with a sensory neuron-like pharmacology,suggested that it may transduce ATP-evoked nociceptor activation. If ATP, and more specifically, P2X3 purinoceptors are involved in nociception, then the development of an antagonist selective for P2X3 could prove useful in pain relief. Lack of P2X3 in other tissues than sensory ganglia could afford a degree of specificity, leading to fewer side effects. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot:1ug/ml Immunohistochemistry (paraffin):1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
