Paired Immunoglobulin-Like Type 2 Receptor beta, Recombinant, Mouse (PILRb)
Artikelnummer:
USB-P2150-50E
Hersteller Artikelnummer:
P2150-50E
Alternativnummer:
USB-P2150-50E-50
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Applikation:
WB
PILR-Beta (paired immunoglobulin-like type 2 receptor-beta) is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-alpha and it likely arose through PILR-alpha gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-Beta and -alpha should not be confused with the similarly named PIRs (also paired immunoglobulin-like receptors), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig-like domains in their extracellular region, PILR-Beta and -alpha show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-Beta is a 196 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a short, eight aa cytoplasmic region. The ECD shows a V-type Ig-like domain (aa 39-135), while the TM segment contains a positively-charged Lys at position 202. This Lys is known to interact with the transmembrane signaling adaptor protein DAP12, making PILR-Beta an activating receptor. Activation of PILR-Beta through CD99 ligation induces NK cell cytotoxicity and dendritic cell secretion of NO and TNF-alpha (3). Mouse PILR-Beta is found on NK cells, neutrophils, macrophages, and monocyte-derived dendritic cells (3). Mouse PILR-Beta ECD is 44% and 75% aa identical to human and rat PILR-Beta ECD, respectively, it is 75% aa identical to the ECD of mouse PILR-alpha (3). Evidence suggests that mouse PILR-Beta will not be active in a human system (3). Potential isoforms of PILR-Beta have been reported. One shows an alternate start site at Met14, a second shows a 21 aa substitution for the C-terminal 67 aa, and a third exhibits multiple polymorphisms for an overall aa identity of 86% (4, 5, 6). Source: A DNA sequence encoding the extracellular domain of mouse PILR-Beta (Ala 15-Gly 180, Accession NP_573472) was fused to a 6x histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0. Definition: Measured by its ability to bind rmCD99/mFc. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 19.6kD migrates as an ~ 39-44kD protein in SDS-PAGE under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
