PAK2, CT (p21 Activated Kinase 2, p21-activated Kinase 2, p21(CDKN1A)-activated Kinase 2, PAK-2, CB422, Gamma PAK, hPAK65, p65-PAK, PAK65, PAKgamma, S6/H4 Kinase, Serine/threonine Protein Kinase PAK 2) (Biotin), Rabbit
Artikelnummer:
USB-P2488-12P-BIOTIN
Hersteller Artikelnummer:
P2488-12P-Biotin
Alternativnummer:
USB-P2488-12P-BIOTIN-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
WB
Immunogen:
14 amino acid peptide sequence near the carboxy terminus of human PAK2.
PAK2 is a ubiquitously expressed, 64kD, serine/threonine protease found at higher levels in skeletal muscle, ovary, thymus and spleen. p21-activated kinases (PAKs) bind activated Cdc42 and Rac via a Cdc42/Rac interactive binding (CRIB) domain. Group 1 PAK kinases (PAK1, 2 and 3) regulate the activity of an adjacent conserved autoinhibitory domain and are associated with cellular processes, including cytoskeleton rearrangement, apoptosis and MAP kinase signalling. Group 2 PAKs (PAK4, 5, and 6) lack the autoinhibitory domain and are not activated by Cdc42/Rac binding. PAK2 has a cytostatic effect on cells and is activated in response to cellular stresses such hyperosmolarity, serum starvation, contact inhibition and DNA damage from radiation and chemicals. Unlike other PAKs, PAK2 can be activated by proteolytic cleavage to generate a constitutively active fragment, PAK2p34, which induces a cell death response. This induction is thought to be regulated by Rho GTPase-activating protein 10 which inhibits the protein kinase activity of PAK2p34 and causes it to localise to the perinuclear region instead of the nucleus. PAK2 may also play a role in X-linked mental retardation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
