Source: Vero cells Inactivation: Inactivated using gamma radiation. Purity: Optimally infected monolayers are harvested, disrupted by sonication and subjected to low speed centrifugation. The clarified cell lysate is pooled with supernatant from the infected culture and concentrated using crossflow ultrafiltration. The resulting antigen preparation contains a high concentration of virus and viral components as well as some cellular material suspended in MEM. Sonication prior to use is suggested. Concentration: ~1mg/ml Form: Supplied as a liquid, lysate in MEM. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Optimally infected monolayers are harvested, disrupted by sonication and subjected to low speed centrifugation. The clarified cell lysate is pooled with supernatant from the infected culture and concentrated using crossflow ultrafiltration. The resulting antigen preparation contains a high concentration of virus and viral components as well as some cellular material suspended in MEM. Sonication prior to use is suggested.
Formulierung:
Supplied as a liquid, lysate in MEM.
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