PauI (BsePI)

Produktübersicht

• Artikelname: PauI (BsePI)
• Artikelnummer: USB-P3113-83
• Hersteller Artikelnummer: P3113-83
• Alternativnummer: USB-P3113-83-200,USB-P3113-83-1000
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-G C G C G C-3 3-C G C G C G-5 Concentration: 10u/ul Source: Paracoccus alcaliphilus ZVK3-3 Buffer: 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used. Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 120-fold overdigestion (7.5u/ug lambda DNA x 16 hours) with PauI. Ligation Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with PauI, more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.06uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Blue White Cloning Assay: The mix of pUC57/HindIII, pUC57/Eco32I and pUC57/PstI digests was incubated with 10 units of enzyme for 16 hours. After religation and transformation 0.2% of white colonies were detected. Methylation Effect: Blocked by CG methylation. Stability during Prolonged Incubation: A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 80C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: AflIII, AscI, DsaI, MluI Number of Recognition Sites in DNA: Lambda: 6 PhiX174: 1 M13mp18/19: 0 pBR322: 0 pUC18/19: 0 pUC57: 0 pTZ19R/U: 0 pBluescriptIIKS(-/+): 2 pBluescriptIISK(-/+): 2 pACYC177: 0 pACYC184: 0 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer.