PDK1 (PDPK1, PI-P3 Dependent Kinase, 3a Phosphoinositide-dependent Protein Kinase 1), Rabbit
Artikelnummer:
USB-P3123-09
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| Artikelname: | PDK1 (PDPK1, PI-P3 Dependent Kinase, 3a Phosphoinositide-dependent Protein Kinase 1), Rabbit |
| Artikelnummer: | USB-P3123-09 |
| Hersteller Artikelnummer: | P3123-09 |
| Alternativnummer: | USB-P3123-09-100 |
| Hersteller: | US Biological |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, WB |
| Immunogen: | A synthetic peptide, RKIQEVWRQRYQS, corresponding to amino acids 301-312 of PDK-1. Accession number AC005591. |
| PDK-1 (3-Phosphoinositide-dependent Protein Kinase-1) phosphorylates AGC kinases. PDK-1 activates conventional PKC and PKCz (zeta) through phosphorylation of critical threonine residues in the activation loop. PDK-1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase-3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:750 Western Blot: 1:750 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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