| Purified pectinase is a multi-component preparation highly effective in depolymerizing plant pectins with varying degrees of esterification. Important enzymatic activities include pectin lyase (EC 4.2.2.10) and activities on polygalacturonic acid (pectin) and nonmethylated polygalacturonic acid (pectate). Contains substantial hemicellulase, cellulase, pectinesterase and xylanase activities which together with pectin lyase and polygalacturonase work synergistically to digest plant cell wall tissues. Purified pectinase has been developed specifically for use in plant protoplast culture studies. The enzyme is assayed using a method which quantitatively measures the liberation of D-galacturonic acid from polygalacturonic acid. This method is based upon determination of reducing sugars using a neocuprine hydrochloride color reagent assay. When used with purified cellulase, purified pectinase has been found to be useful for generating good yields of viable protoplast in several plant systems, e.g., corn, soybean, red beet, sunflower, tomato and citrus. In general, a concentration range of 0.1% to 0.5% pectinase (with accompanying 0.5% to 1.5% cellulase) used at 24C to 37C for periods of 1 to 16 hours will yield good results. CAS No: 9033-35-6 Source: Aspergillus niger Purity: Chromatographically purified. Form: Supplied as a dialyzed, lyophilized powder Assay Method: One unit liberates 1 umole of D-galacturonic acid from polygalacturonic acid per minute at 37deg.C, pH 5.0. Storage and Stability: Lyophilized powder may be stored at 4C. Stable for 6 months after receipt at 4C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
