PHD1-3, pan, Human, Control Peptide (HIF-prolyl Hydroxylase 1-3)

Produktübersicht

• Artikelname: PHD1-3, pan, Human, Control Peptide (HIF-prolyl Hydroxylase 1-3)
• Artikelnummer: USB-P3373B
• Hersteller Artikelnummer: P3373B
• Alternativnummer: USB-P3373B-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: ELISA

Produktbeschreibung

Oxygen is absolutely critical for the survival of mammalian cells. Hypoxia induced factor (HIF) is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. There are 3-types of alpha subunits (HIF1-3alpha) and one HIF-beta subunits. However, the three HIF-alpha subunits are regulated by oxygen in a similar fashion, i.e. by regulated stabilization of the alpha-subunits. Under normal conditions, HIF-alpha Prolines are hydroxylated at Pro-402 and Pro-564. This allows binding of von Hippel-Lindau (VHL), the substrate recognition component of the E3 ubiquinated ligase complex, subsequent ubiquitination and degradation of HIF-alpha by the proteasome. Under hypoxic conditions, hydroxylation of HIF-alpha is inhibited and this prevents HIF-alpha degradation. The enzymes responsible for HIF-hydroxylation are known as HIF-prolyl hydroxylases (PHD1-3 or HPH1, HPH2, and HPH3). The three PHDs have been identified to hydroxylate the motif, LXXLAP* with *P being the hydroxyproline. Source: 18aa peptide of Human PHD2/EGLN1 (1). This peptide is significantly conserved in PHD1-3 in mouse, rat, and human. Location: ~N-terminus Applications: Suitable for use in ELISA as a control peptide for P3373A. Other applications not tested. Recommended Dilution: ELISA: Use 50-100ng of control peptide/well Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Purified
• Formulierung: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.