Phosphatase, Alkaline, Calf Intestine, Solution (~3000u/mg), CAS [[9001-78-9]]
Artikelnummer:
USB-P4071-03
Hersteller Artikelnummer:
P4071-03
Alternativnummer:
USB-P4071-03-1,USB-P4071-03-5,USB-P4071-03-10
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Alkaline Phosphatase is used to dephosphorylate dsDNA and RNA in molecular cloning and DNA sequencing reactions. Source: Calf Intestine Prepared from US origin starting material and collected in USDA approved facilities Protein Concentration: 1mg/ml Assay Method: Based on the method of Garen and Levinthal whereby the reaction velocity is derived by measuring the absorbance increase at 410nm after hydrolysis of p-nitrophenyl phosphate. Form: Supplied as a liquid in deionized water. Activity: ~3000 units/mg protein (glycine units). 1 glycine unit = 2-3 DEA units Unit Definition: One unit of enzyme hydrolyzes 1umole of p-nitrophenyl phosphate per minute at 37C, pH 9.8 in the presence of Mg and Zn. Quality Control: SDS-PAGE No contaminating RNase, DNase or Proteases detected Storage and Stability: Stable for 1 year at 2-8C
CAS Nummer:
[9001-78-9]
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