Selective phosphoinositol 3-kinase SH3 ligand Sequence (linear): H2N-Arg-Lys-Leu-Pro-Pro-Arg-Pro-Arg-Arg-OH Phosphoinositide 3 kinase (PI3K) is a kinase catalyzing the phosphorylation of PI(4,5)P2 at the D3 position of the inositol ring. The resulting product PI(3,4,5)P3 can be recognized by PH domain-containing signaling molecules, including various kinases and their regulators, and activates the corresponding cell signaling pathways, eventually leading to cell growth, survival and cellular movement (1). PI3K is a heterodimer with a p85 regulatory subunit and p110 catalytic subunit. The p85 subunit has two SH2 domains binding to phospho-tyrosine with the consensus motif (Y*XXM) where X denotes any amino acid and Y* is phosphorylated tyrosine. This motif exists in many of the receptor tyrosine kinases and their substrates, such as EGFR, FGFR, IRS-1, BCAP and GAB1 (2,3). The docking of PI3K in the membrane vicinity through p85 recognization of the phosphorylated YXXM in signaling molecules activates the PI3K pathway. Phospho-(Tyr) p85 PI3K Binding Motif Antibody specifically recognizes PI3K binding targets at their phosphorylated YXXM motif, providing a powerful new tool for PI3K binding protein discovery and characterization as well as HTS drug screening for potential regulators. Amino Acid Analysis: Confirms expected sequence Peptide Content: 81.2 4% Solubility: H2O Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Stable for 12 months at -20C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
