PLA2R1 also called muscle (M-)type PLA2R or secretory (s)PLA2R is a 180-200kD type I transmembrane glycoprotein that is a member of subgroup VI of the C-type lectin superfamily. The 1487aa mouse PLA2R1 contains a 26aa signal sequence, a 1370 aa ECD, a 21aa transmembrane sequence, and a 70aa cytoplasmic domain with an endocytosis motif. The ECD contains one ricin B-type lectin domain aa42-165, a fibronectin type II region aa176-224, and eight C-type lectin carbohydrate recognition domains CRDs, aa241-1377. CRDs 3-5 are the major PLA2 binding site. The mouse PLA2R1 ECD shares 75% and 86% aa identity with human and rat PLA2R1, respectively. One isoform shows a 35aa N-terminal extension, and a potentially secreted isoform contains a 10aa substitution for aa680-1487. Matrix metalloproteinases can generate a 180kD soluble inhibitory form in mouse serum that retains PLA2 binding activity. Mouse PLA2R1 is widely expressed, including on type II alveolar cells, neutrophils and mast cells, and binds mouse secretory PLA2s IB, IIA, IIE, IIF and X, and snake venom PLAs. Among mammals, PLA2R1 ligand recognition is mainly species-specific. PLA2R1 can be inflammatory by inhibiting PLA2-induced release lipid mediators such as arachadonic acid. PLA2R1 can also mediate internalization and clearance of PLA2s. PLA2R1 can also be pro-inflammatory, and its deletion in mouse reduces susceptibility to LPS-induced endotoxic shock. Engagement activates the MAPK/ERK signaling pathway and enhances pro-inflammatory cytokine release. PLA2 binding can stimulate cell adhesion, proliferation or senescence, neutrophil elastase release, and smooth muscle contraction. All PLA2R1 intracellular signaling is largely independent of ligand phospholipase activity. Source: Recombinant corresponding to Gln27-Gly1392 from mouse PLA2R1 expressed in mouse myeloma cell line, NS0. Molecular Weight: ~157.5kD Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Human PLA2G1B is immobilized at 5ug/ml (100ul/well), the concentration of Recombinant Mouse PLA2R1 that produces 50% of the optimal binding response is found to be ~1.5-7.5ug/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
