Phosphoserine Phosphatase, Recombinant, Human (PSP)

Produktübersicht

• Artikelname: Phosphoserine Phosphatase, Recombinant, Human (PSP)
• Artikelnummer: USB-P4076-95
• Hersteller Artikelnummer: P4076-95
• Alternativnummer: USB-P4076-95-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Human Phosphoserine phosphatase (hPSP) is an important enzyme in the phosphorylated pathway of serine biosynthesis, which contributes a major portion of the endogenous L-serine. Similar to known L-3-phosphoserine phosphatases, it catalyzed the Mg2+-dependent hydrolysis of L-phosphoserine and an exchange reaction between L-serine and L-phosphoserine. Recently, its complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the a-helical bundle domain, is important to substrate recognition and hydrolysis. Recombinant human hPSP was overexpressed in E. coli. It consists of 225 amino acids. Sequence: MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AMGGAVPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKIIMIGDG ATDMEACPPA DAFIGFGGNV IRQQVKDNAK WYITDFVELL GELEE Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 25
• Reinheit: 95% by SDS-PAGE. Purified by using conventional chromatography techniques.
• Formulierung: Supplied as a liquid in 20mM HEPES, pH 7.5, 1mM DTT, 100mM potassium chloride.