Plasminogen prescursor or profibrinolysin or plasma trypsinogen (human 810aa, chromosome 6q26) is synthesized in the kidney. It is present in plasma and many other extracellular fluids. Plasminogen is the zymogen in the circulating blood from which plasmin is formed. Plasminogen is a single-chain glycoprotein with 790 amino acid residues (MW ~90-94kD). Various isoelectric forms exist and can be separated by means of isoelectric focusing (IEF). Native plasminogen (glu-plasminogen) has a N-terminal glutamic acid group. Activation to the active form, plasmin, by urokinase involves cleavage at the arg-val bond between residues 560 and 561, resulting in the formation of the 2-chain plasmin molecule held together by 2 disulfide linkages. The heavier chain contains about 411 residues and the lighter chain about 233. The main function of plasmin is the digestion of fibrin in blood clots. Plasmin is a proteolytic enzyme with a specificity similar to that of trypsin. Like trypsin, plasmin belongs to the family of serine proteinases, in which the active site catalytic triad, his-57, asp-102, and ser-195 (chymotrypsin numbering), is situated in the light chain. The mature chain represents 20-810 aa. Processed active peptide domain is 20-97 aa. Angiostatin mature chain represents 98-810 aa. This regions contains 5 structurally related kringles domains K1-5 (Kringle 1, 103-181 aa, Kringle 2, 184-262 aa, Kringle 3, 275-352 aa, Kringle 4, 377-454 aa, Kringle 5, 481-560 aa). Each Kringle domain contains 6 conserved cysteines. Kringles 1-5 domains share approx 45-50% sequence homology. Applications: Suitable for use in ELISA or for antigenic applications in immunological protocols. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht:
92
Reinheit:
98% by SDS PAGE. No contaminants detected. Single band by SDS PAGE, IEP, and/or RID.
Formulierung:
Supplied as a lyophilized powder from 0.02M Tris buffer, pH 7.5 with 2mM EDTA. Reconstitute with 1ml sterile ddH2O.
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