Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis. Plasminogen prescursor or profibrinolysin or plasma trypsinogen (human 810 aa, chromosome 6q26) is synthesized in the kidney. It is present in plasma and many other extracellular fluids. Plasminogen is the zymogen in the circulating blood from which plasmin is formed. Plasminogen is a single-chain glycoprotein with 790 amino acid residues. Activation to the active form, plasmin, by urokinase involves cleavage at the arg-val bond between residues 560 and 561, resulting in the formation of the 2-chain plasmin molecule held together by 2 disulfide linkages. The heavier chain contains about 411 residues and the lighter chain about 233. The main function of plasmin is the digestion of fibrin in blood clots. Plasmin is a proteolytic enzyme with a specificity similar to that of trypsin. Like trypsin, plasmin belongs to thefamily of serine proteinases, in which the active site catalytic triad, his-57, asp-102, and ser-195 (chymotrypsin numbering), is situated in the light chain. The mature chain represents 20-810 aa. Processed active peptide domain is 20-97 aa. Angiostatin mature chain represents 98-810 aa. This regions contains 5 structurally related kringles domains K1-5 (Kringle 1, 103-181 aa, Kringle 2, 184-262 aa, Kringle 3, 275-352 aa, Kringle 4, 377-454 aa, Kringle 5, 481-560 aa). Each Kringle domain contains 6 conserved cysteines. Kringles 1-5 domains share approx 45-50% sequence homology. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: Heat once and load 10ul/lane and visualize with appropriate antibodies. This preparation is intended for qualitative purpose and not to serve as standard of known concentration. Plasminogen protein is ~90kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified (95%) from human plasma protein that has been shown by certified tests to be negative for HbsAg and HIV and HCV.
Formulierung:
Supplied in SDS-PAGE sample buffer (reduced).
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