Human Plk3 (GenBank Accession No. NM_004073), (a.a. 58-339) with N-terminal HIS6 tag, MW 36.7kD, expressed in an Baculovirus infected Sf9 cell expression system. Purity: >80% by SDS-PAGE. Specific Activity: 500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1nmol of casein substrate per minute at pH 7.4 and 30C Assay buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 2mg/ml casein, 0.1mM ATP and 0.1ug/ml Plk3. Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
