| Tween-20 is a polysorbate surfactant whose stability and relative non-toxicity allow it to be used as a detergent and emulsifier in a number of domestic, scientific, and pharmacological applications. It is a polyoxyethylene derivative of sorbitan monolaurate, and is distinguished from the other members in the Tween range by the length of the polyoxyethylene chain. The commercial product contains a range of chemical species. Biological Applications: Tween 20 has a broad range of applications. For example, it is used: to stabilize purified protein derivative (PPD) solution, used in skin testing for tuberculosis exposure, as a blocking agent in immunologic assays, such as Western blots and ELISA and as a solubilizing agent of membrane proteins for lysing mammalian cells, at a concentration of 0.05 to 0.5%. CAS No: 9005-64-5 Synonyms: Polysorbate 20, Polyethylene glycol sorbitan monolaurate Appearance: Yellow to amber viscous liquid. Hydroxyl Number: 96-108 Water (KF): 3% Specific Gravity (25C): 1.080-1.120 Acid Value: 2.2mg KOH/g Saponification Value: 50mg KOH/g Residual Solvents (1, 4-Dioxane): 0.001% RNase, DNase (1.5% and 3%): None Detected Storage and Stability: Store at RT. |
