Profilin 2, Recombinant, Human (Profilin-2, Profilin II, Profilin-II, PFN2, PFN-2, D3S1319E, PFL)
Artikelnummer:
USB-P9005-89W
Hersteller Artikelnummer:
P9005-89W
Alternativnummer:
USB-P9005-89W-50
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Applikation:
WB
PFN2 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein is ubiquitously expressed with highest expression in kidney, brain and skeletal muscle. Like other members of the profilin family, PFN2 functions as an actin monomer-binding protein that influences the structure of the cytoskeleton by regulating actin polymerization in response to extracellular signals. At high concentrations, it prevents the polymerization of actin whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Recombinant PFN2 protein, fused to His-tag at N-terminus, was expressed in E. coli. Source: Recombinant corresponding to aa1-140 of human PFN2 6x His tagged expressed in E. coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MAGWQSYVDN LMCDGCCQEA AIVGYCDAKY VWAATAGGVF QSITPIEIDM IVGKDREGFF TNGLALGAKK CSVIRDSLYV DGDCTMDIRT KSQGGEPTYN VAVGRAGRVL VFVMGKEGVH GGGLNKKAYS MAKYLRDSGF Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
