Protein A and Protein G bind specifically to Fc regions of many mammalian immunoglobulins. Protein A and Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants, and other biological fluids. These reagents are also commonly used to capture immune complexes generated in immunoprecipitation experiments. Because Protein A and Protein G vary widely in their abilities to bind individual immunoglobulins, consult the table below before proceeding with your application. It should be noted that the Fc binding characteristics of naturally produced Protein A and Protein G are identical to their recombinant counterparts. Protein-A/Sepharose ratio: ~2.7mg Protein A per ml beads Binding capacity: ~22mg Rabbit IgG per ml beads Protein A Molecular Weight: 42kD (native) Protein A Ig Binding Sites: 4 per molecule Albumin Binding Sites/molecule: none Storage and Stability: May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C. Freezing will damage or destroy its performance.
Reinheit:
~98% (SDS-PAGE)
Formulierung:
Supplied as a 50% suspension (5 ml Protein A-Sepharose 4B + 5 ml PBS) in 10 mM phosphate-buffered saline, pH 7.4, containing 0.1% Proclin
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