Protein Expression Media, Power Broth(TM) (Powder) pEX(TM)

Produktübersicht

• Artikelname: Protein Expression Media, Power Broth(TM) (Powder) pEX(TM)
• Artikelnummer: USB-P9102-40C
• Hersteller Artikelnummer: P9102-40C
• Alternativnummer: USB-P9102-40C-500
• Hersteller: US Biological
• Kategorie: Biochemikalien

Produktbeschreibung

Historically, E. coli has been cultivated in LB Broth. Many gene expression protocols recommend this medium. LB Broth was developed in the 1950s, nearly 20 years before the first gene was cloned and 30 years before recombinant protein expression became routine. LB Broth has proved very useful for cultivating E. coli. It was not specifically designed with the intention of maximizing the expression of recombinant proteins. LB Broth is not normally supplemented with a carbon source, nor is it buffered. Thus, the growth yields that one can obtain with LB are limited. Many proteins are not readily expressed in LB. These blends of media have proven to be the most widely applicable formulations showing consistently superior performance over the traditional medium, LB Broth. The optimum conditions for the expression of a recombinant protein requires attention to four culture-related parameters: strain employed, the medium composition, the time course of induction, and the concentration of inducing agent. Power Broth(TM) is a proprietary medium formulation developed by for improved expression of recombinant proteins in E. coli. This media are composed of a complex, rich mixture of amino acids, vitamins, and a carbon source at higher levels than Terrific Broth. Not sure which media expression system to choose? United States Biological has developed a unique research tool for protein expression media selection: Media Optimization Kit(TM) (P9102-40): Other unique, proprietary media formulations: pEX(TM) Protein Expression Media Media Optimization Kit(TM) Turbo Broth(TM) Superior Broth(TM) Power Broth(TM) Hyper Broth(TM) M9Y (Glucose) Broth(TM) Glucose-Nutrient Mix LB Broth (Miller) Appearance: Light brown to brown, homogenous, free flowing powder Solubility (52g/L): Yellow-amber, clear, complete pH: ~6.7 Directions per Liter: 1. Dissolve 52g in 1 liter of deionized water. 2. Add 4ml of glycerol to the Power Broth solution. 3. Dispense at desired volume into appropriate bottles or flasks. 4. Autoclave at 121C for 15-20 min, depending on the volume per container. Allow to cool. 5. Add sterile antibiotics as needed. Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.