Protein Expression Media, HyperBroth(TM) (Powder) pEX(TM)

Produktübersicht

• Artikelname: Protein Expression Media, HyperBroth(TM) (Powder) pEX(TM)
• Artikelnummer: USB-P9102-40D
• Hersteller Artikelnummer: P9102-40D
• Alternativnummer: USB-P9102-40D-500
• Hersteller: US Biological
• Kategorie: Biochemikalien

Produktbeschreibung

Hyper Broth(TM) is a proprietary medium formulation for improved expression of recombinant proteins in E. coli. The medium is a complex, rich formulation which supplies amino acids, vitamins, and glucose at high levels. It is made without the use of animal-derived raw materials and is APF Certified. The medium is buffered at pH 7.3 0.2, which permits high glucose levels. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 4-5 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. Plasmid copy numbers are higher than for LB, which can facilitate protein expression. This medium is suitable as the base for fermentation cultures and is excellent for feed-batch applications. Biomass yields will depend on the process and the strain employed. Hyper Broth(TM) powder is supplied with P9102-40F: Protein Expression Media, Glucose-Nutrient Mix, M9Y (Powder) pEX(TM) Appearance: Light to medium brown, homogenous, free flowing powder Solubility (44.5g/L): Tan to amber, complete pH: 7.3 0.3 pEX(TM) Protein Expression Media P9102-40: Media Optimization Kit(TM) P9102-40A: Turbo Broth(TM) P9102-40B: Superior Broth(TM) P9102-40C: Power Broth(TM) P9102-40D: Hyper Broth(TM) P9102-40E: M9Y (Glucose) Broth(TM) P9102-40F: Glucose-Nutrient Mix P9102-40G: LB Broth (Miller) Not sure which media expression system to choose? United States Biological has developed a unique research tool for protein expression media selection: Media Optimization Kit(TM) Our experiences with the expression of recombinant proteins have shown that the level of expression is very much dependent on the medium used. Some proteins are expressed well in one medium, but are not expressed in the same host strain in another medium or protein may be expressed in one medium while another protein is not expressed in the same medium. This demonstrates why for each recombinant protein a medium screen becomes a crucial part of the optimization process. To facilitate the researchers efforts to develop the best systems for their proteins, we have developed a Media Optimization Kit.(TM). The kit is composed of six media formulations (including four that are unique and proprietary) which we have found over the years to be the most reliable for the increased production of recombinant proteins in Escherichia coli. The kit is used to quickly and easily identify the most suitable medium for the expression of a particular recombinant protein. Included are suggested approaches for improving the production of recombinant proteins, thus passing along the knowledge our staff scientists have acquired while working to produce a wide variety of proteins. Also Available: P9102-40D: Protein Expression Media, HyperBroth(TM) (Powder) pEX(TM) P9102-40D1: Protein Expression Media, HyperBroth(TM) (Liquid) pEX(TM)