Pvu I

Produktübersicht

• Artikelname: Pvu I
• Artikelnummer: USB-P9391
• Hersteller Artikelnummer: P9391
• Alternativnummer: USB-P9391-300,USB-P9391-1500
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-C G A T C G-3 3-G C T A G C-5 Concentration: 10u/ul Source: Proteus vulgaris Buffer: 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used. Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25C), 300mM KCl, 1mM DTT, 0.1mM EDTA, 0.02% Triton X-100, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with PvuI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with PvuI, more than 90% of the DNA fragments can be ligated at a 5-termini concentration of 0.03uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Methylation Effects: PvuI does not cut CGATm5CG. Blocked by CG methylation. Stability during Prolonged Incubation: A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Only small amounts of enzyme (up to 10 units) can be inactivated by incubation at 80C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: Bsh1285I, SgfI, PacI Number of Recognition Sites in DNA: Lambda: 3 PhiX174: 0 M13mp18/19: 1 pBR322: 1 pUC18/19: 2 pUC57: 2 pTZ19R/U: 2 pBluescriptIIKS(-/+): 2 pBluescriptIISK(-/+): 2 pACYC177: 2 pACYC184: 0 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-CpoI fragments in 1 hour at 37C in 50ul of assay buffer.