PKLR, Control Peptide (Pyruvate Kinase Isozymes R/L, Pyruvate Kinase 1, R-type/L-type Pyruvate Kinase, Red Cell/Liver Pyruvate Kinase, PK1, PKL)
Artikelnummer:
USB-P9545-03G
Hersteller Artikelnummer:
P9545-03G
Alternativnummer:
USB-P9545-03G-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Applikation:
ELISA
Control peptide for P9545-03F. Glycolysis occurs in the cytosol, yielding 2 ATP, 2 pyruvate, and 2 NADH + 2 H+ from each glucose molecule. The first two steps of glycolysis convert glucose-6-phosphate into a form that is easily cleaved into phosphorylated three-carbon units. PKLR: rat, mouse, human: 574aa each - 62.3kD, Mouse chromosome: 3F1. Highly expressed in liver and RBCs. The PKLR gene codes for both the liver and red blood cell isozymes. The PKM2 gene, located on chromosome 15q22, encodes 2 muscle-specific isoforms, M1 and M2. The PK enzyme functions as a homotetramer. Studies with the rat enzyme suggested that the red cell type is longer than the liver type in the 5-prime terminal end, but the rest of the sequence is very similar. deduced protein sequence was similar to that of L-type PK, with an additional 31aa at the N terminus. Addiitonal studies demonstrated that the difference between the L- and R-type pyruvate kinases is due to tissue-specific mRNAs, likely resulting from differential processing of a common nuclear RNA precursor. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Source: Synthetic peptide corresponding to 17aa peptide of human PKLR (accession P30613), N-terminal. Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: ELISA: 5-30ug control peptide per 1ug of aff pure IgG or 1ul antiserum Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
