RAF, Recombinant, Human, GST-Tag (A Raf, A-raf-1, ARaf Proto-oncogene Serine/threonine Protein Kinase, ARAF1, ARAF, Oncogene PKS2, PKS, PKS2, Proto-oncogene Pks, RAFA1, Ras-binding Protein DA-Raf, v-Raf Mouse Sarcoma 3611 Viral On
Artikelnummer:
USB-R0495-05A
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| Artikelname: | RAF, Recombinant, Human, GST-Tag (A Raf, A-raf-1, ARaf Proto-oncogene Serine/threonine Protein Kinase, ARAF1, ARAF, Oncogene PKS2, PKS, PKS2, Proto-oncogene Pks, RAFA1, Ras-binding Protein DA-Raf, v-Raf Mouse Sarcoma 3611 Viral On |
| Artikelnummer: | USB-R0495-05A |
| Hersteller Artikelnummer: | R0495-05A |
| Alternativnummer: | USB-R0495-05A-10 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues. Recombinant protein corresponding to human A-RAF, aa282-606 (end), with N-terminal GST tag and C-terminal His tag, containing the mutations YY301-302DD, expressed in Baculovirus infected Sf9 cell expression system. Specific Activity: 40pmol/min/ug Assay Conditions: 40mM Tris, 10mM MgCl2, M2865-06J (5ug), 1uM ATP and 4-8ng/ul R0495-05A at 30C for 30 minutes. ATP reduction is detected using Kinase-Glo Luminescent Kinase Assay Platform. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. |
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