Raf 1 RBD, Recombinant, Human (Agarose)
Artikelnummer:
USB-R0495-15
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| Artikelname: | Raf 1 RBD, Recombinant, Human (Agarose) |
| Artikelnummer: | USB-R0495-15 |
| Hersteller Artikelnummer: | R0495-15 |
| Alternativnummer: | USB-R0495-15-600 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors. Human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli. Uniprot/Accession: NM_001085049.1 Applications: Suitable for use in Ras affinity precipitation. Other applications not tested. Recommended Dilutions: Ras affinity precipitation: 5-10ug. Precipitates Ras-GTP from HeLa lysates that were loaded in vitro with GTPgS and EGF-stimulated HeLa cells that were lysed in a magnesium containing lysis buffer. Note: Due to spontaneous deactivation of Ras, use lysates that have been stored at -70C for less than 1 month. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Also Available Ras Products: Enzymes: R0495-15: Raf 1 RBD, Recombinant, Human (Agarose) R1198-20: Ras, GST (Agarose) Antibodies: R1198: Ras, p21, N,H,K Mab Mo xHu Kits: R1198-07: Ras Activation Assay Kit, BioAssay(TM) Ha-Ras Products: Antibodies: R1198-34: Ras, H (Harvey Murine Sarcoma Virus Oncogene, Ha-Ras) Mab Rb xHu RasGAP Products: Antibodies: R1198-11: Ras, GAP (Ras GTPase Activating Protein, GAP) Mab Mo xHu R1198-12: Ras, GAP (Ras GTPase Activating Protein, GAP) Pab Rb xHu |
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