Human Ret (GenBank Accession No. NM_020975), (aa658-end) with N-terminal GST tag, expressed in an Baculovirus infected Sf9 cell expression system. Specific Activity: 40 U/ug. One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30C. Assay Buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Naorthovanadate, 0.1mM ATP, 30ug/ml Poly (Glu:Tyr) 4:1 substrate and 2ug/ml recombinant cMet. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
