Riboflavin kinase, Recombinant Human (RIFK, RP11-422N19.2, RFK)
Artikelnummer:
USB-R2001-02
Hersteller Artikelnummer:
R2001-02
Alternativnummer:
USB-R2001-02-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Riboflavin kinase, also known as flavokinase, belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. It is an enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN). Recombinant riboflavin kinase , expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant human Riboflavin kinase, 1aa-162, His-tagged, expressed in E. coli AA Sequence: MGSSHHHHHH SSGLVPRGSH MPRADCIMRH LPYFCRGQVV RGFGRGSKQL GIPTANFPEQ VVDNLPADIS TGIYYGWASV GSGDVHKMVV SIGWNPYYKN TKKSMETHIM HTFKEDFYGE ILNVAIVGYL RPEKNFDSLE SLISAIQGDI EEAKKRLELP EHLKIKEDNF FQVSKSKIMN GH Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
