RNA Ligase T4, 10-20u/ul

Produktübersicht

• Artikelname: RNA Ligase T4, 10-20u/ul
• Artikelnummer: USB-R2041
• Hersteller Artikelnummer: R2041
• Alternativnummer: USB-R2041-1000
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Purified from an E.coli strain carrying a T4 RNA ligase overproducing plasmid. Catalyses the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5-phosphate and 3-hydroxyl termini of single-stranded RNA or DNA. The minimal substrate is a nucleoside 3, 5-diphosphate. Applications: RNA 3-end labelling (1). Joining RNA to RNA (2). Circularization of oligonucleotides (3). Joining synthetic oligodeoxyribonucleotides (4). Specific modifications of tRNAs (5). Oligodeoxyribonucleotide ligation to single-stranded cDNAs (6). Generation of composite primers to PCR (7). Quality Control: Tested for the absence of exo- and endodeoxyribonucleases and ribonucleases. Concentration: 10-20u/ul. Note: The concentration of ATP in the reaction mixture depends upon the type of ligation reaction (1-7). The final concentration of BSA is usually 0.01-0.1mg/ml (1-7). Note: The concentration of ATP in the reaction mixture depends upon the type of ligation reaction (1-7). The final concentration of BSA is usually 0.01-0.1mg/ml (1-7). Unit Definition: 1 unit of enzyme catalyses the conversion of 1 nanomole of 5-[32P]-(rA)12-18 to a phosphatase-resistant form in 30 minutes at 37C. Activity Assay: 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 10mM DTT, 1mM ATP, 10uM 5-[32P]-(rA)12-18 (10uM in 5-termini). Storage Buffer: 10mM Tris-HCl (pH 7.5), 1mM DTT, 50mM KCl, 0.1mM EDTA and 50% glycerol. Supplied with: R2041A: RNA Ligase T4, 10-20u/ul, 10X Buffer R2041B: RNA Ligase T4, 10-20u/ul, ATP Buffer R2041C: RNA Ligase T4, 10-20u/ul, BSA Buffer Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.