The (Ro) SS-A antigen is comprised or an acidic 60kD protein that may also be associated with a RNA ranging in size from 80 to 112 bases (1). The antigenic activity of the (Ro) SS-A antigen appears to be independent of the RNA since the precipitin activity remains after treatment with RNase or separation from the RNA (1). Anti-(Ro) SS-A autoantibodies were described originally as precipitating autoantibodies in sera of Sjogrens Syndrome and Systemic Lupus Erythematosus patients. The presence of autoantibodies against the (Ro)SS-A antigen may be used as a diagnostic aid. Elevated levels of Anti-(Ro) SS-A have been detected in as high as 96% of Sjogrens Syndrome patients (2), 49% of patients with Systemic Lupus Erythematosus (2), 83% of mothers of infants with isolated complete congenital heartblock or Neonatal Lupus Dermatitis, and over 75% of patients with Subacute Cutaneous Lupus. Recently, a strong correlation between low positive levels of Anti-(Ro) SS-A and subdinical dry eyes or mouth was found in a substantial portion of an elderly population (3). In addition, it has been shown that 70% of patients with (Ro) SS-A precipitating antibodies also have rheumatoid factor (4). The titer of rheumatoid factor and the concentration of Anti-(Ro) SSA have been closely correlated in Primary Sjogrens Syndrome (4). Recent developments in ELISA methodology have increased the sensitivity and the ability of the diagnostician to quantitate patient antibody titers. Activity: 1000u/ml Unit Definition: One unit of activity is the amount of antigen that will generate an absorbance of 1.2 when assayed using standard procedure for ELISA. Activity will vary with assay conditions/ protocols. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by researcher. Storage and Stability: Aliquot and store at -70+/-10C. Aliquots are stable for 6 months after receipt at 70+/-10C. Avoid freeze-thaw cycles. Note: Product is unstable above 0C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Also Available: R2997: Ro/SS-A Antigen Pab Hu xHu R2997-04: Ro/SS-A Antigen
Reinheit:
Purified by immunoaffinity chromatography using immobilized anti-SSA-A (Ro) immunoglobulins. Other antigens (Sm, RNP, Scl-70, Jo-1) are not detected in ELISA. Homogeneity is verified by SDS PAGE.
Formulierung:
Supplied as a liquid in 0.01M Tris, 0.53M sodium chloride, 0.02% sodium azide.
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