Securin, Recombinant, Human (Cut2, ESP1-associated Protein 1, EAP1, hPTTG, MGC126883, MGC138276, Pds1, Pituitary Tumor Transforming 1, PTTG, PTTG1, TUTR1)
Artikelnummer:
USB-S0379-80B
Hersteller Artikelnummer:
S0379-80B
Alternativnummer:
USB-S0379-80B-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
PTTG1, also known as Securin, is primarily involved in the regulation of sister chromatid separation during cell division. It has two identified roles, the first one is to help the transport of separase(cysteine protease) to the nucleus and the second role is to inhibit the catalytic activity of separase. It is ubiquitinated by the Anaphase Promoting Complex (APC), and then degraded by the Proteasome, releasing separase. Recombinant PTTG1 protein , expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant human PTTG1, aa1-202, His-tagged, expressed in E. coli AA Sequence: MGSSHHHHHH SSGLVPRGSH MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA KSSVPASDDA YPEIEKFFPF NPLDFESFDL PEEHQIAHLP LSGVPLMILD EERELEKLFQ LGPPSPVKMP SPPWESNLLQ SPSSILSTLD VELPPVCCDI DI Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
