SHP 1, Recombinant, Human, aa243-541 (SHPTP-1, SH2 Containing Protein Tyrosine Phosphatase 1, PTP1C, SH-PP1, HCP, SHP, PTPN6)

Artikelnummer: USB-S1013-28
Artikelname: SHP 1, Recombinant, Human, aa243-541 (SHPTP-1, SH2 Containing Protein Tyrosine Phosphatase 1, PTP1C, SH-PP1, HCP, SHP, PTPN6)
Artikelnummer: USB-S1013-28
Hersteller Artikelnummer: S1013-28
Alternativnummer: USB-S1013-28-100,USB-S1013-28-250
Hersteller: US Biological
Kategorie: Molekularbiologie
The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). Additional amino acid (Met) is attached at N-terminus. Recombinant protein corresponding to aa243-541 from human, expressed in E. coli. Accession: NP_002822 Amino Acid Sequence: MGFWEEFESL/ QKQEVKNLHQ/ RLEGQRPENK/ GKNRYKNILP/ FDHSRVILQG/ RDSNIPGSDY/ INANYIKNQL/ LGPDENAKTY/ IASQGCLEAT/ VNDFWQMAWQ/ ENSRVIVMTT/ REVEKGRNKC/ VPYWPEVGMQ/ RAYGPYSVTN/ CGEHDTTEYK/ LRTLQVSPLD/ NGDLIREIWH/ YQYLSWPDHG/ VPSEPGGVLS/ FLDQINQRQE/ SLPHAGPIIV/ HCSAGIGRTG/ TIIVIDMLME/ NISTKGLDCD/ IDIQKTIQMV/ RAQRSGMVQT/ EAQYKFIYVA/ IAQFIETTKK/ KLEVLQSQKG/ QESEYGNITY Specific Acitivity: 8000unit/mg Unit Definition: One unit will hydrolyze 1nanomole of p-nitrophenyl-phosphatate (pNPP) per minute at pH 7.4, 37C. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht: 34.3
NCBI: 002822
UniProt: P29350
Reinheit: 95% by SDS PAGE. Endotoxin: 1EU/ug (LAL)
Formulierung: Supplied as a liquid in 25mM Tris-HCl, pH 7.5, 2mM beta-mercaptoethanol, 1mM EDTA, 1mM DTT, 20% glycerol