The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies). Additional amino acid (Met) is attached at N-terminus. Recombinant protein corresponding to aa243-541 from human, expressed in E. coli. Accession: NP_002822 Amino Acid Sequence: MGFWEEFESL/ QKQEVKNLHQ/ RLEGQRPENK/ GKNRYKNILP/ FDHSRVILQG/ RDSNIPGSDY/ INANYIKNQL/ LGPDENAKTY/ IASQGCLEAT/ VNDFWQMAWQ/ ENSRVIVMTT/ REVEKGRNKC/ VPYWPEVGMQ/ RAYGPYSVTN/ CGEHDTTEYK/ LRTLQVSPLD/ NGDLIREIWH/ YQYLSWPDHG/ VPSEPGGVLS/ FLDQINQRQE/ SLPHAGPIIV/ HCSAGIGRTG/ TIIVIDMLME/ NISTKGLDCD/ IDIQKTIQMV/ RAQRSGMVQT/ EAQYKFIYVA/ IAQFIETTKK/ KLEVLQSQKG/ QESEYGNITY Specific Acitivity: 8000unit/mg Unit Definition: One unit will hydrolyze 1nanomole of p-nitrophenyl-phosphatate (pNPP) per minute at pH 7.4, 37C. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.