Heterogeneous nuclear ribonucleoproteins (hnRNP) bind to nascent RNA polymerase II transcripts and are involved in transcript-specific packaging as well as alternative splicing of pre-mRNAs. hnRNPs also appear to influence mRNA metabolism and transport. In vivo, the M proteins bind to mRNA, and in vitro they bind avidly to poly(G) and poly(U) homopolymers. Four different isoforms of hnRNP are produced by alternative splicing: M1, M2, M3, and M4. Ongoing studies have theorized that hnRNPs may cause certain genetic diseases such as myotonic dystrophy, which is the most common form of adult muscular dystrophy. The identification of hnRNPs may therefore be important to determining the cause and cure for genetic diseases. Applications: Suitable for use in ELISA. Other applications have not been tested. Recommended Dilutions: ELISA: 0.5-1ug/ml coating concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
90% (SDS-PAGE), Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in 20mM Tris-HCI, 0.32M sodium chloride, 10mM magnesium chloride, 0.2mM DTT, pH 7.4, 0.1mM PMSF, 0.1% bromo-nitro-dioxane/ methylisothiazoline, 20% glycerol.
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