Smad Ubiquitination Regulatory Factor-1, N-terminal FLAG-Tag, Recombinant, Human (Smad Ubiquitination Regulatory Factor 1, Smurf1, E3 Ubiquitin Ligase SMURF1, HGNC:16807, KIAA1625, Smad-specific E3 Ubiquitin Ligase 1, SMAD-specifi
Artikelnummer:
USB-S1014-60R
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| Artikelname: | Smad Ubiquitination Regulatory Factor-1, N-terminal FLAG-Tag, Recombinant, Human (Smad Ubiquitination Regulatory Factor 1, Smurf1, E3 Ubiquitin Ligase SMURF1, HGNC:16807, KIAA1625, Smad-specific E3 Ubiquitin Ligase 1, SMAD-specifi |
| Artikelnummer: | USB-S1014-60R |
| Hersteller Artikelnummer: | S1014-60R |
| Alternativnummer: | USB-S1014-60R-20 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| Source: Recombinant corresponding to aa150-end of human SMURF 1, fused with N-terminal FLAG tag expressed in a Baculovirus infected Sf9 cell expression system (NM_020429). Specific Activity: 0.0055pmol/min/ug Assay conditions: 50ul reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5ug), E2 (2.8ug), Biotin-Ubiquitin (0.5ug)and Smurf1 (0-1ug) in 50mM Tris pH 7.4) incubate for 30min at 37C, then add to streptavidin coated plates and incubate for 30min at 37C. Add antibody against Smurf1, incubate 1 hour. Finally, incubate 1 hour with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (lambda-exc=330 nm, lambda-em=620 nm). Applications: Suitable for use in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70C. Aliquots are stable for at least 6 months at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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