Sorbitol Dehydrogenase, aa1-357, Recombinant, Human (SDH, SORD, SORD1, L Iditol 2 Dehydrogenase, L-iditol 2-dehydrogenase)
Artikelnummer:
USB-S5350-31
Hersteller Artikelnummer:
S5350-31
Alternativnummer:
USB-S5350-31-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
SORD, also known as L-iditol 2-dehydrogenase or SORD1, is a 357aa member of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney and in the lens of the eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses. Source: Recombinant corresponding to aa1-357 of human SORD 6x his tagged, expressed in E.coli. AA Sequences: MGSSHHHHHH SSGLVPRGSH MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP Enzyme Activity: Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze D-fructose to D-sorbitol per minute at pH 7.5 at 37C. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
