SPHK2, Long Form, Recombinant, Human (Sphingosine kinase 2, SK 2)

Produktübersicht

• Artikelname: SPHK2, Long Form, Recombinant, Human (Sphingosine kinase 2, SK 2)
• Artikelnummer: USB-S5455-04H
• Hersteller Artikelnummer: S5455-04H
• Alternativnummer: USB-S5455-04H-10
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Human Sphingosine kinase 2 ( GenBank Accession No.NM_020126), full length with N-terminal His tag, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: 20 U/ug. Sphingosine kinase activity was determined using D-erythrosphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min. Assay Buffer: 50mM HEPES, pH 7.4, 150mM NaCl, 5mM MgCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 4uM D-erythrosphingosine, and 0.75ug/ml sphingosine kinase 2. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Produkteigenschaften

• Molekulargewicht: 74
• NCBI: 020126
• UniProt: P08571
• Reinheit: 80% by SDS-PAGE
• Formulierung: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween 20, 50% glycerol and 3mM DTT.