SREBP-1 (Sterol Regulatory Element Binding Protein-1, SREBP1, ADD1, D630008H06, Sterol Regulatory Element Binding Transcription Factor 1, SREBF1), Rabbit
Artikelnummer:
USB-S6505-15A
Hersteller Artikelnummer:
S6505-15A
Alternativnummer:
USB-S6505-15A-25,USB-S6505-15A-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IF, IHC, WB
Immunogen:
Synthetic peptide corresponding to a portion between aa300-400 of human SREBP. Cellular Localization: Membranes of ER, Golgi and Cytoplasmic Vesicles. Processed SREBP1 (aa1-490.) translocates to the nucleus. Species Sequence Homology: Chinese Hamster, 92%
SREBP-1 and-2 are transcription factors which participate in the control of cholesterol homeostasis. SREBPs proteins are attached to the endoplasmic reticulum and nuclear envelope. They are proteolytically cleaved and activated in response to conditions of low cellular sterol. Upon activation of SREBP-1 or -2, an ~480-500aa, N-terminal cleavage fragment of these proteins enters the nucleus, activating transcription of enzymes and other proteins required for cholesterol synthesis. SCA (SREBP-cleavage activity) and caspase-3 proteases cleave SREBPs. SREBP proteins containing point mutations at caspase-3 cleavage sites (Asp460 in SREBP-1 and Asp468 in SREBP-2) do not become cleaved following induction of apoptosis. This suggests that SREBPs may play some role in apoptotic processes. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500. Detects a band at ~65kD. Immunohistochemistry (Frozen and paraffin): 1:200-1:500 Immunofluorescence (IC): 1:200 Optimal dilutions to be determined by the researcher. Positive Control: Mouse liver whole tissue lysate Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
