ST2, NT (Suppressor of Tumorigenicity 2, ST2L, ST2V, DER4, Homolog of Mouse Growth Stimulation-expressed, Interleukin 1 Receptor-like 1, IL1RL1, Interleukin 1 Receptor-related Protein, Lymphocyte Antigen 84, Ly84, T1 Protein), Rab
Artikelnummer:
USB-S6606-50B
Hersteller Artikelnummer:
S6606-50B
Alternativnummer:
USB-S6606-50B-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA, IF, IHC, WB
Immunogen:
Snthetic peptide corresponding to 16aa at the N-terminus of mouse ST2. Epitope: aa40-90. Species Sequence Homology: equine (74%) and bovine (67%)
ST2 is a member of the Toll-interleukin 1 receptor (TIR) superfamily, expressed by mast cells and TH2 helper cells, but not by TH1 effector cells. The majority of TIR superfamily members, which includes the Interleukin-1 receptor (IL-1R1), Toll-like receptors (TLRs) and the cytosolic adaptor proteins MyD88, TIRAP, Trif and TRAM, initiate innate immunity through the ultimate activation of the transcription factor NF-kappaB and are inducers of the inflammatory response. In contrast ST2, which itself is unable to activate NF-kappaB, has been identified as a negative regulator of IL-1R1 and TLR4 signaling by sequestering MyD88 and TIRAP (1), in line with its role as a crucial receptor in TH2-cell mediated responses (2). Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 1-5ug/ml. Require antigen retrieval by heat mediation with a citrate buffer (pH6). Immunofluorescence: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
