SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. The human SCF gene encodes for a 273 amino acid transmembrane protein, which contains a 25 amino acid N-terminal signal sequence, a 189 amino acid extracellular domain, a 23 amino acid transmembrane domain, and a 36 amino acid cytoplasmic domain. The secreted soluble form of SCF is generated by proteolytic processing of the membrane anchored precursor. Recombinant mouse SCF is an 18.3kD polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF. Amino Acid Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of the human TF-1 cells is 10 ng/ml, corresponding to a specific activity of 1x10e5units/mg. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O and carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
98% (SDS-PAGE and HPLC). Endotoxin: 0.1ng/ug (1EU/ug)
Formulierung:
Supplied as a lyophilized powder from 10mM sodium phosphate, pH 6.5. Reconstitute in sterile dH2O to 0.1-1mg/ml, which can then be further diluted to other aqueous solutions.
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