Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:500-1:1000. Optimal dilutions to be determined by the researcher. Ratio of AP to Streptavidin (molar): ~1.0 Activity: 600 units/mg conjugate. One unit=1 micromole of p-nitrophenol liberated from p-nitrophenylphosphate per minute at 37C (pH 9.8). Storage and Stability: Store product at 4C. DO NOT FREEZE! Stable at 4C for 6 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Formulierung:
Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, 0.08% sodium azide, 1mM Mg++, 0.1mM Zn++, pH 7.5. Labeled with Alkaline Phosphatase (AP).
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